Premium
Glycosylated Hemoglobin in Dogs: Precision, Stability, and Diagnostic Utility
Author(s) -
Easley J. Roger
Publication year - 1986
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1986.tb00861.x
Subject(s) - hemoglobin , coefficient of variation , medicine , whole blood , reference range , significant difference , endocrinology , chemistry , chromatography
Summary The precision and stability or the ion exchange chromatography assay for canine glycosylated hemoglobin (HbA 1 ) were examined. The coefficient of variation (CV) of within‐run replicate assays was 1.3 to 2.6%; the CV of between‐run duplicate assays was 3.1%. The mean HbA 1 content in 44 healthy dogs was 7.1% (SD = 1.1%, range = 5.1–9.7%). Paired aliquots of 12 blood samples were stored at 4° and 25°, and HbA 1 was measured on the day of collection and at 3, 5, and 7 days aftercollection. In the blood stored at 4°, no significant increase in the HbA 1 content was seen. No significant increase in HbA 1 content was found in the blood stored at 25° after 3 days, but dramatic increases were observed after 5 and 7 days of storage. No significant difference was observed in the HbA 1 content in heart blood collected 18 hours after death from 9 dogs kept at 25°C. The HbA 1 content was measured in 10 hospitalized diabetic dogs. Five of the dogs had received no insulin and all 5 had elevated HbA, values. The other 5 dogs had received insulin for 1 to 9 months; 2 of the 5 had increased HbA 1 content. The HbA 1 content was determined periodically for 9 months in one diabetic dog and it declined from 14.0% to 8.2%.