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Mean Platelet Volume Artifacts: The Effect of Anticoagulants and Temperature on Canine Platelets
Author(s) -
Handagama Prem,
Feldman Bernard,
Kono Constance,
Farver Thomas
Publication year - 1986
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1986.tb00651.x
Subject(s) - mean platelet volume , platelet , filopodia , volume (thermodynamics) , thrombus , scanning electron microscope , chemistry , limiting , medicine , materials science , cell , biochemistry , composite material , physics , quantum mechanics , mechanical engineering , engineering
Summary Improper handling of specimens results in artifactually high Mean Platelet Volume (MPV) measurements limiting their usefulness as a clinical tool. MPV measurement and Scanning Electron Microscopy (SEM) were performed on split specimens collected from normal dogs using two anticoagulants and two temperatures over a period of 4 hours. Platelets exposed to EDTA and maintained at 4 °C (39.2 °F) exhibited the highest artifactual increase in MPV, while those exposed to citrate and maintained at 37°C (98.6 °F) exhibited minimal change. The increase in MPV was accompanied by platelet shape change from a smooth disc to an irregular sphere with filopodia. It is recommended that citrated specimens maintained at 37°C be used in all MPV measurements.