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A Sensitive Autoradiographic Procedure for Factor VIII‐Related Antigen in Canine Plasma
Author(s) -
Schlink Gerald T.,
Johnson Gary S.
Publication year - 1983
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1983.tb00618.x
Subject(s) - antigen , precipitin , antibody , stain , microbiology and biotechnology , pathology , chemistry , staining , biology , immunology , medicine
Summary A second‐antibody autoradiographic technique was found to be suitable for visualization of precipitin arcs produced by the elec‐troimmunoassay or crossed immunoelectro‐phoresis of factor Vlll‐related antigen in canine plasma. With the new autoradiographic procedure the electroimmunoassay was approximately ten times more sensitive than with the commonly used protein stain, Co‐omassie Blue R 250. Unlike earlier autoradiographic techniques, second‐antibody au‐toradiography requires only commercially available reagents.