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Determination of Canine Factor VIII‐related Antigen Using Commercial Antihuman F VIII Serum
Author(s) -
Johnstone I.B.,
Crane S.
Publication year - 1980
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1980.tb00891.x
Subject(s) - antigen , microbiology and biotechnology , immunology , chemistry , virology , medicine , biology
Summary Factor Vlll‐related antigen (F Vlll:AGN) in 21 canine plasma samples was assayed by immunoelectrophoresis using a rabbit anti‐canine F VIII serum prepared from a canine F VIII concentrate and a commercial rabbit antihuman F VIII serum. A good correlation existed (r value 0.916) between the antigen levels obtained using the two sera. In normal dogs the plasma F VIII. AG/V level was 95 ± 39% (Mean ± SD) compared to 175 ± 40% in dogs with severe hemophilia A and 17 ± 15% in dogs with von Willebrand's disease. It was concluded that there was sufficient cross reactivity between canine F VIII and commercial rabbit antihuman F VIII serum to make the latter useful in the differential diagnosis of F VIII deficiencies in the dog.