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Determination of the Concentration of Protein in Cerebrospinal Fluid With A New Dye‐Binding Method
Author(s) -
Barsanti J.A.,
Duncan J.R.
Publication year - 1978
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/j.1939-165x.1978.tb00789.x
Subject(s) - coomassie brilliant blue , trichloroacetic acid , cerebrospinal fluid , chromatography , chemistry , staining , pathology , medicine
SUMMARY The protein concentration of cerebrospinal fluid (CSF) was measured on 50 samples with a new dye‐binding method using Coomassie Brilliant Blue G‐250 (CBB). The results of this method correlated well (r = 0.99) with those of the trichloroacetic acid‐Ponceau S method (TCA‐PS). Since the CBB method involves only one step, it is recommended as a simple method of determining protein concentration in CSF.