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Impact of a new refrigerator on the preservation of hepatic grafts
Author(s) -
Kanamoto Mami,
Shimada Mitsuo,
Utsunomiya Tohru,
Imura Satoru,
Morine Yuji,
Ikemoto Tetsuya,
Mori Hiroki,
Hanaoka Jun
Publication year - 2012
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/j.1872-034x.2012.00987.x
Subject(s) - cold storage , viaspan , economic shortage , transplantation , liver transplantation , orthotopic liver transplantation , refrigerator car , group b , group a , chemistry , medicine , andrology , surgery , biology , horticulture , engineering , mechanical engineering , linguistics , philosophy , government (linguistics)
Aim: Current medical transplantation methods focus on solutions for major problems such as the shortage of donors. To overcome these issues, expanding organ preservation time has become a major concern. A new refrigerating chamber has been recently developed, which can cool the inside of a material to the required temperature by frequently sensing the temperature of both inside and surface of the materials. The purpose of this study is to evaluate the usefulness of a new refrigerating system in hepatic preservation. Methods: The liver grafts were harvested from rats and divided into two groups. Group A consisted of grafts preserved in chilled University of Wisconsin solution (UW) solution (on ice) for 24, 72 and 168 h. Group B consisted of grafts preserved in the UW solution in a new refrigerator at 4°C. Results: In group B, aspartate aminotransferase released into effluent after cold storage for 72 h showed a marked decrease compared to group A ( P < 0.05). The levels of ammonia and lactate decreased significantly in group B ( P < 0.05). In group B, the levels of adenosine triphosphate were significantly preserved after cold storage for 24 h and 72 h compared to group A ( P < 0.05). Immunohistochemistry showed positive cells for heme oxygenase‐1 were significantly increased in group B after cold storage. Conclusion: This new refrigerator can improve preservation injury of hepatic grafts and may provide an innovative technique for liver transplantation.