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Crucial role of impaired Kupffer cell phagocytosis on the decreased Sonazoid‐enhanced echogenicity in a liver of a nonalchoholic steatohepatitis rat model
Author(s) -
Yoshikawa Shohei,
Iijima Hiroko,
Saito Masaki,
Tanaka Hironori,
Imanishi Hiroyasu,
Yoshimoto Naoki,
Yoshimoto Tomohiro,
FutatsugiYumikura Shizue,
Nakanishi Kenji,
Tsujimura Tohru,
Nishigami Takashi,
Kudo Atsushi,
Arii Shigeki,
Nishiguchi Shuhei
Publication year - 2010
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/j.1872-034x.2010.00670.x
Subject(s) - phagocytosis , kupffer cell , in vivo , flow cytometry , parenchyma , pathology , microbubbles , mononuclear phagocyte system , intravital microscopy , echogenicity , liver disease , immunostaining , medicine , biology , immunology , ultrasound , immunohistochemistry , radiology , microbiology and biotechnology
Aims: To evaluate the dynamics of Kupffer cell (KC) phagocytosis by performing both in vivo and in vitro studies using Sonazoid (GE Healthcare, Oslo) in a rat nonalcoholic steatohepatitis (NASH) model. Methods: Contrast enhanced ultrasonography (CEUS) was performed on a rat NASH model induced by a methionine choline deficient diet (MCDD) and control rats, and Sonazoid was used to measure the signal intensity in the liver parenchyma. The uptake of Sonazoid by the KCs was observed by intravital microscopy. Their phagocytic capability was evaluated in vitro using isolated and cultured KCs. The uptake of fluorescein isothiocyanate (FITC)‐labeled latex beads was observed and quantitatively analyzed by flow cytometry. Results: In the MCDD group, liver parenchymal enhancement was reduced 20 min after the Sonazoid injection. Microscopic observation of the isolated and cultured KCs revealed that the number of phagocytosed Sonazoid microbubbles was significantly decreased. Confocal laser scanning microscopic (CLSM) observation showed a decrease in the uptake of the latex beads. A decreased phagocytic capacity in the MCDD group was suggested by the quantitative analysis using flow cytometry, as well as by intravital microscopy. Conclusions: CEUS with Sonazoid is a powerful evaluation tool to diagnose NASH from an early stage of the disease.