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Clinical application of an enzyme‐linked immunosorbent assay detecting hepatoma‐specific γ‐glutamyltransferase
Author(s) -
Wang NianYue,
Zhang Dai,
Zhao Wei,
Fang GuoXiang,
Shi YuLing,
Duan MuHao
Publication year - 2009
Publication title -
hepatology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.123
H-Index - 75
eISSN - 1872-034X
pISSN - 1386-6346
DOI - 10.1111/j.1872-034x.2009.00538.x
Subject(s) - hepatocellular carcinoma , medicine , gamma glutamyltransferase , receiver operating characteristic , gastroenterology , pathology , enzyme , biology , biochemistry
Aim: To develop a sandwich enzyme‐linked immunosorbent assay (ELISA) measuring hepatoma‐specific datura stramonium agglutinin–tightly bounding γ ‐glutamyltransferase (DSA‐GGT) and evaluate its clinical application for hepatocellular carcinoma (HCC) diagnosis. Methods: Serum DSA‐GGT concentrations were measured with the sandwich ELISA system in 96 patients with HCC, 240 patients with chronic liver diseases and 119 healthy subjects. The diagnostic performance of DSA‐GGT for HCC was assessed using receiver operating characteristic (ROC) curves. The diagnostic accuracy of DSA‐GGT was compared with serum α‐fetoprotein (AFP). Results: The area under the ROC curve of DSA‐GGT in discriminating patients with HCC from non‐HCC was 0.865 (95% confidence interval: 0.818–0.915, P < 0.001). Serum DSA‐GGT was positive in 67 out of 96 patients with HCC and 23 out of 240 patients with non‐HCC diseases. The sensitivity and specificity of DSA‐GGT and AFP for the diagnosis of HCC were 69.8% and 90.5%, and 72.9% and 89.1%, respectively. A higher sensitivity (93.8%) in the identification of HCC was observed by combining DSA‐GGT and AFP. Conclusion: The sandwich ELISA system showed good reliability and reproducibility, and using the measurement, we found that serum DSA‐GGT was a valuable marker of HCC, as a usable complementary to AFP. The sensitivity for identifying HCC could be significantly improved by combining DSA‐GGT and AFP, and the combination could be used in large‐scale screening for HCC in susceptible individuals.