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Associations Between Anti‐Microbial Resistance Phenotypes, Anti‐Microbial Resistance Genotypes and Virulence Genes of Escherichia coli Isolates from Pakistan and China
Author(s) -
Yaqoob M.,
Wang L. P.,
Wang S.,
Hussain S.,
Memon J.,
Kashif J.,
Lu C.P.
Publication year - 2013
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2012.01360.x
Subject(s) - virulence , biology , microbiology and biotechnology , tetracycline , genotype , gene , antibiotic resistance , cefotaxime , ampicillin , escherichia coli , genetics , antibiotics
Summary The objective of this study was to determine the association between phenotypic resistance, genotypic resistance and virulence genes of Escherichia coli isolates in Jiangsu province, China and Punjab province Pakistan. A total of 62 E. coli isolates were characterized for phenotypic resistance, genotypic resistance and virulence factor genes. The anti‐microbial resistance phenotype and genotypes in relation to virulence factor genes were assessed by statistical analysis. Of 20 tested virulence genes, twelve were found and eight were not found in any isolates. sitA and TspE4C2 were the most prevalent virulence genes. Of the 13 anti‐microbial agents tested, resistance to ampicillin, sulphonamide and tetracycline was the most frequent. All isolates were multiresistant, and 74% were resistant to trimethoprim and sulphamethaxazole. Phenotypically, tetracycline‐, cefotaxime‐ and trimethoprim‐resistant isolates had increased virulence factors as compared with susceptible isolates. Genotypically, resistant genes Tem, ctx‐M, Tet, Sul 1, dhfr1, Cat2 and flo‐R showed the association with the virulence genes. Almost all classes of anti‐microbial‐resistant genes have a high association with virulence. Resistant isolates have more virulent genes than the susceptible isolates.