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Detection of Lumpy Skin Disease Virus Antigen and Genomic DNA in Formalin‐Fixed Paraffin‐Embedded Tissues from an Egyptian Outbreak in 2006
Author(s) -
Awadin W.,
Hussein H.,
Elseady Y.,
Babiuk S.,
Furuoka H.
Publication year - 2011
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2011.01238.x
Subject(s) - outbreak , histology , polymerase chain reaction , biology , virology , pathology , virus , antigen , immunohistochemistry , medicine , immunology , biochemistry , gene
Summary An outbreak of lumpy skin disease (LSD) was reported in 2006 in Egypt affecting 16 provinces. Biopsies and post‐mortem tissue samples were collected from calves that showed typical clinical signs of LSD and fixed in formalin. These samples were collected from a private dairy farm in the Damietta province of Egypt. Formalin‐fixed paraffin‐embedded tissue samples were assessed using histology, and skin lesions were classified as either acute or subacute/chronic. Both lumpy skin disease virus (LSDV) DNA detected by polymerase chain reaction and LSDV antigen detected by immunohistochemistry using a capripoxvirus‐specific monoclonal antibody were observed in the acute skin lesions and in some subacute/chronic skin lesions.