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Molecular Typing of Canine Parvovirus Variants by Polymerase Chain Reaction and Restriction Enzyme Analysis
Author(s) -
Kumar M.,
Nandi S.
Publication year - 2010
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2010.01167.x
Subject(s) - canine parvovirus , polymerase chain reaction , restriction enzyme , biology , virology , parvovirus , typing , striated muscles , microbiology and biotechnology , virus , gene , genetics , anatomy
Summary Canine parvovirus type 2 (CPV‐2) is a pathogen of dogs, which causes acute gastroenteritis and lymphopenia mostly in young pups. This paper reports the incidence of CPV‐2 infection in diarrhoeic dogs with an aim to define the involvement of various variants of canine parvovirus circulating in India. CPV‐2a, a variant of CPV‐2 was differentiated from CPV‐2b by polymerase chain reaction (PCR). The samples positive for CPV‐2b were further analysed by PCR and restriction endonuclease (RE) analysis using Mbo II to detect the CPV‐2c variant. Of 129 faecal samples studied, 78 were found positive for canine parvovirus by PCR. Among the 78 samples, 27 were of CPV‐2a, 39 of CPV‐2b and 12 of CPV‐2c type, respectively. This study also showed that CPV‐2c, a new variant, is circulating in India. The CPV‐2c could be successfully detected by PCR and RE analysis while CPV‐2b is the major antigenic type prevalent in this region followed by CPV‐2a.