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Development of a Loop‐mediated Isothermal Amplification (LAMP) Assay for Rapid Diagnosis of Babesia canis infections
Author(s) -
Müller H.,
Aysul N.,
Liu Z.,
Salih D. A.,
Karagenc T.,
Beyer D.,
Kullmann B.,
Ahmed J. S.,
Seitzer U.
Publication year - 2010
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2010.01113.x
Subject(s) - babesia canis , rhipicephalus sanguineus , canis , dermacentor reticulatus , subspecies , loop mediated isothermal amplification , biology , vector (molecular biology) , tick , virology , veterinary medicine , zoology , ixodidae , ecology , genetics , medicine , dna , gene , recombinant dna
Summary Vector‐borne diseases are rising in interest due to global warming, which is believed to impact on the distribution of vectors into new areas thus influencing the occurrence and epidemiology of vector‐borne pathogens. Babesia canis belongs to the Piroplasmidae and there are three described subspecies, namely B. canis canis , B. canis rossi and B. canis vogeli . They are each transmitted by a different tick‐species, Dermacentor reticulatus , Haemaphysalis leachi and Rhipicephalus sanguineus , respectively. There are also differences in the geographical distribution and pathogenicity to dogs of each subspecies. In this study, we aimed to establish a rapid and easy to perform DNA‐based test using loop‐mediated isothermal amplification to detect all three Babesia canis subspecies in one assay.