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Detection of BPV‐1 and ‐2 and Quantification of BPV‐1 by Real‐Time PCR in Cutaneous Warts in Cattle and Buffaloes
Author(s) -
Pangty K.,
Singh S.,
Goswami R.,
Saikumar G.,
Somvanshi R.
Publication year - 2010
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2009.01096.x
Subject(s) - biology , real time polymerase chain reaction , bovine papillomavirus , sybr green i , papilloma , pathology , veterinary medicine , microbiology and biotechnology , medicine , gene , genetics , genome
Summary Bovine cutaneous warts (CWs) were investigated in Northern India. Of 49 cases, 44 were recorded in cattle and 5 in buffaloes. These animals had mild to moderate grade infections. Grossly, cases of CWs appeared to be of exophytic type, however, different types of growth patterns were observed. A total of 26 biopsies (cattle 21 and buffaloes 5) from CWs‐affected animals studied histopathologically were diagnosed as exophytic and cauliflower‐like fibropapilloma 13, exophytic and dome‐shaped fibropapilloma 5, occult and/or fibroblastic type papilloma 3, cauliflower‐like papilloma 3, endophytic fibropapilloma 1 and fibroma 1. On PCR analysis, 11 CWs and 2 normal skin samples showed BPV‐1, ‐2 mixed infections. A rapid, sensitive and reliable real‐time SYBR Green PCR test to detect BPV‐1, BPV‐2 and to quantify BPV‐1 was developed. Results of amplification and dissociation plot of real‐time PCR revealed that six samples were BPV‐1 positive, eight were BPV‐2 positive and six were positive for both BPV‐1 and ‐2. CWs samples from different dairy farms testing positive for BPV‐1 by PCR assay were also positive using Quantitative real‐time SYBR Green PCR assay. For the first time, mixed infection of BPV‐1 and ‐2 was detected in India and BPV‐1 load was quantified by real‐time SYBR Green PCR assay.