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Analysis of Antigenic Cross‐Reactivity Between Subgroup C Avian Pneumovirus and Human Metapneumovirus by Using Recombinant Fusion Proteins
Author(s) -
Luo L.,
Sabara M. I.,
Li Y.
Publication year - 2009
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2009.01085.x
Subject(s) - human metapneumovirus , virology , recombinant dna , metapneumovirus , polyclonal antibodies , biology , antibody , antigen , fusion protein , paramyxoviridae , cross reactivity , western blot , glycoprotein , microbiology and biotechnology , virus , gene , respiratory system , immunology , cross reactions , viral disease , genetics , respiratory tract infections , anatomy
Summary Avian pneumovirus subgroup C (APV/C) has recently been reported to be more closely related to human metapneumovirus (hMPV) as determined by sequence analysis. To examine the antigenic relationship between APV/C and hMPV, the APV/C fusion (F) gene was cloned and expressed as an uncleaved glycoprotein in a baculovirus system. The reactivity of the APV/C F protein with antibodies against APV subgroups A, B, C, and hMPV was examined by Western blot analysis. The results showed that the expressed APV/C F protein was not only recognized by APV/C‐specific antibodies but also by antibodies raised against hMPV. Previously expressed recombinant hMPV F protein also reacted with APV/C‐specific antibodies, suggesting that there was significant antigenic cross‐reactivity and a potential evolutionary relationship between hMPV and APV/C. Interestingly, the recombinant F proteins from APV/C and hMPV were not recognized by polyclonal antibodies specific to APV subgroups A and B.