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Enhancement of Peripheral Blood CD8 + T Cells and Classical Swine Fever Antibodies by Dietary β ‐1,3/1,6‐glucan Supplementation in Weaned Piglets
Author(s) -
Wang Z.,
Shao Y.,
Guo Y.,
Yuan J.
Publication year - 2008
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2008.01049.x
Subject(s) - immune system , beta glucan , cd8 , antibody , medicine , lymphocyte , biology , white blood cell , glucan , immunology , endocrinology , zoology , biochemistry
Summary The experiment was conducted to evaluate the effect of dietary supplementation with 0, 50, 100 and 200 mg/kg of β‐1,3/1,6‐glucan extracted from Saccharomyces cerevisiae on cellular immune responses and classical swine fever virus (CSFV) antibody production of weaned piglets. Thirty‐two (Landrace × Large White; 26 days old; 7.05 ± 0.25 kg body weight) castrated male piglets were randomly allocated to four treatments, eight pigs per treatment. All piglets were vaccinated with live‐attenuated Chinese strain cell vaccines at 26 days of age. The results showed that dietary supplementation with β‐1,3/1,6‐glucan could significantly ( P < 0.01) enhance peripheral blood lymphocyte proliferation activity in weaned piglets after 14 days administration, whereas no significant effect ( P > 0.05) was observed on day 28. Dietary β‐1,3/1,6‐glucan supplementation could significantly enhance CSFV antibody blocking rate ( P < 0.05) on days 14 and 21 after inoculation. Although dietary β‐1,3/1,6‐glucan supplementation did not significantly influence ( P > 0.05) lymphocyte subgroups on day 14, high levels of β‐1,3/1,6‐glucan (100 and 200 mg/kg) increased the percentage of cytotoxic/suppressor lymphocytes (CD8 + ) significantly ( P < 0.05), decreased the ratio of CD4 + /CD8 + significantly ( P < 0.05) and increased the percentage of total T lymphocytes (CD3 + ) on day 28. These results suggest that β‐1,3/1,6‐glucan may have a beneficial effect on the immune system of swine, and dietary β‐1,3/1,6‐glucan supplementation had a transient impact on lymphocyte proliferation activity. The CSFV antibody blocking rate and the percentage of peripheral blood CD8 + T cells could be enhanced by continuous β‐1,3/1,6‐glucan supplementation. These findings support the potential application of β‐1,3/1,6‐glucan as a prophylactic agent in increasing animal immune functions and controlling CSF disease.