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Techniques Developed in China for Foot‐and‐Mouth Disease Diagnosis
Author(s) -
Lu Z.,
Cao Y.,
Bao H.,
Qi S.,
Guo J.,
Shang Y.,
Jiang T.,
Zhang Q.,
Ma J.,
Liu Z.,
Liu X.,
Yin H.,
Xie Q.
Publication year - 2008
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/j.1865-1682.2008.01027.x
Subject(s) - virology , serology , foot and mouth disease virus , foot and mouth disease , multiplex , virus , antibody , african horse sickness , typing , antigen , medicine , biology , immunology , microbiology and biotechnology , bioinformatics
Summary Over the past several decades, a series of new methods have been developed in China for the diagnosis of foot‐and‐mouth disease (FMD). These methods are theoretically consistent with Office International des Epizooties standards for FMD diagnosis and include: antigen‐capture ELISA and colloid‐gold test strip for viral antigen typing, liquid‐phase blocking ELISA (LPBE) for detection of antibodies against O and Asia 1 FMDV, and an indirect ELISA for detection of antibodies against the non‐structural protein (NSP) 3ABC (3ABC‐I‐ELISA). Several molecular diagnostic methods have also been developed for detection of fragments of the FMDV genome within viral samples, such as multiplex RT–PCR, typing RT–PCR and real‐time RT–PCR. In China, FMD vaccines are compulsorily used in all livestock. In such a situation, monitoring of the immune status of animal herds and disease surveillance become very important. Liquid‐phase blocking ELISA is routinely used in China for monitoring the immune status, and 3ABC‐I‐ELISA is feasible for large‐scale serological surveys based on differentiation of FMDV‐infected animals from vaccinated animals. Combined use of 3ABC‐ELISA and mRT–PCR is described for survey of FMD virus‐prevalent herds and virus carriers.