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Evaluation of three commercial enzyme‐linked immunosorbent assays for the detection of antibodies against Salmonella spp. in meat juice from finishing pigs in Spain
Author(s) -
Vico J. P.,
Engel B.,
Buist W. G.,
MainarJaime R. C.
Publication year - 2010
Publication title -
zoonoses and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 65
eISSN - 1863-2378
pISSN - 1863-1959
DOI - 10.1111/j.1863-2378.2010.01364.x
Subject(s) - salmonella , herd , serotype , veterinary medicine , biology , population , antibody , food science , zoology , virology , medicine , bacteria , environmental health , immunology , genetics
Summary The control of animal salmonellosis is considered as a major objective in Europe and indirect ELISAs will be important tools for the implementation of control programs for this infection in pigs. We analyse the results yielded by three commercial ELISAs (Herdcheck® Swine Salmonella, SALMOTYPE® Pig Screen, and PrioCHECK® Salmonella) on meat juice samples from a population of slaughter pigs of Aragon, NW Spain, to assess their efficacy using traditional and latent‐class approaches. Overall, the Herdcheck® Swine Salmonella detected more Salmonella ‐infected pigs than the other two tests, but its relative sensitivity was low (65.9%). A similar result was observed when only serotypes detectable by this test were considered (69.1%). When a Bayesian approach was used the Herdcheck® Swine Salmonella showed also the highest overall accuracy (sensitivity = 88% and specificity = 74%). Our results suggest that a relatively small proportion of the observed prevalence in herds would be explained by using these ELISAs. Also, this study points out that when different ELISA tests are used within the same herd, results may differ substantially. Thus, caution is advised if it is decided to use these assays for herd health classification in Spanish Salmonella control programs.