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Virulence Factors and Antimicrobial Susceptibility of Escherichia coli Isolated from Calves in Turkey
Author(s) -
Güler L.,
Gündüz K.,
Ok Ü
Publication year - 2008
Publication title -
zoonoses and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 65
eISSN - 1863-2378
pISSN - 1863-1959
DOI - 10.1111/j.1863-2378.2008.01121.x
Subject(s) - ceftiofur , microbiology and biotechnology , nalidixic acid , biology , escherichia coli , ampicillin , cefoxitin , fimbria , enterotoxigenic escherichia coli , shiga toxin , enrofloxacin , tetracycline , virulence , stx2 , antibiotic resistance , virology , enterotoxin , ciprofloxacin , antibiotics , bacteria , staphylococcus aureus , gene , biochemistry , genetics
Summary Escherichia coli isolates from calves were investigated by multiplex PCR assays for the presence of genes encoding K99, F41, F17‐related fimbriae, heat‐stabile enterotoxin a (STa), intimin ( eae ) and Shiga toxins (s tx 1 and s tx 2). A total of 120 E. coli isolates, 75 isolated from diarrhoeic or septicemic calves and 45 from clinically healthy calves aged between 1 day and 2 months were tested. Each isolate was obtained from different calves in different herds. Among the isolates from diseased animals, 12 (16%) isolates from 1‐ to 7‐day‐old diarrhoeic calves were detected as enterotoxigenic E. coli which possessed K99, F41 and STa in combination; F17‐related fimbriae genes were detected in 33 (44%) isolates and they were found in combination with K99 + F41 + STa in two isolates. Of 120 isolates, 16 carried eae , eight stx 1 and five stx 2 genes alone or in combination. None of the eae ‐ or s tx ‐positive strains was identified as O157:H7. However, results indicate that calves may be carrier of Shiga toxin‐producing E. coli which have potential as a human pathogen. Antimicrobial susceptibility of 75 isolates from diseased calves was determined by agar disk diffusion method for 14 antimicrobial agents. In 77.3% of the isolates, multiresistance was detected. Higher resistance rates were detected for cephalothin (72%), tetracycline (69.3%), kanamycin (69.3%), ampicillin (65.3%), nalidixic acid (53.3%), trimethoprim‐sulphamethoxazole (52%) and enrofloxacin (41.3%), respectively. No resistance was found for ceftiofur and cefoxitin.