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Production and Characterization of Monoclonal Antibodies to poly 100 S1 Protein of Avian Infectious Bronchitis Virus
Author(s) -
Hu J. Q.,
Li Y. F.,
Guo J. Q.,
Shen H. G.,
Zhang D. Y.,
Zhou J. Y.
Publication year - 2007
Publication title -
zoonoses and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 65
eISSN - 1863-2378
pISSN - 1863-1959
DOI - 10.1111/j.1863-2378.2007.01030.x
Subject(s) - epitope , avian infectious bronchitis virus , virology , monoclonal antibody , biology , microbiology and biotechnology , recombinant dna , protein subunit , neutralization , infectious bronchitis virus , glycoprotein , antigen , virus , antigenic variation , antibody , escherichia coli , vero cell , fusion protein , gene , genetics , covid-19 , medicine , disease , pathology , infectious disease (medical specialty)
Summary Fragments within S1 genes ( poly 100 S1) of infectious bronchitis virus (IBV) strains ZJ971, M41 and SC021202 (SC) were subcloned into a prokaryotic expression vector and expressed in Escherichia coli . Monoclonal antibodies (mAbs) against the recombinant poly 100 S1 proteins were produced, characterized and used to analyse epitopes on the S1 subunit of IBV. Nine mAbs raising from the three poly 100 S1 proteins recognized five different epitopes of the S1 subunit, designated as S1‐A, B, C, D and E. Epitopes S1‐C and S1‐D are common for the three IBV strains, while S1‐A and S1‐B exist on ZJ971 and M41 strains, and S1‐E was a strain‐specific epitope for SC strain. Immunocytochemistry indicated that all the mAbs to the poly 100 S1 proteins can react with the homologous S1 glycoprotein expressed in Vero cells. Moreover neutralization test demonstrated that only mAbs 6E2, 4F9 and 6G4 had neutralization activity for the homologous IBV. These mAbs to poly 100 S1 protein were potential candidates for detecting and distinguishing IBV strains, and also used to examine antigenic variation of the S1 protein.