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Application of Different Methods for the Diagnosis of Experimental Paratuberculosis in Goats
Author(s) -
Munjal S. K.,
Tripathi B. N.,
Paliwal O. P.,
Boehmer J.,
Homuth M.
Publication year - 2007
Publication title -
zoonoses and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 65
eISSN - 1863-2378
pISSN - 1863-1959
DOI - 10.1111/j.1863-2378.2007.01006.x
Subject(s) - paratuberculosis , lipoarabinomannan , subclinical infection , ouchterlony double immunodiffusion , antigen , immunodiffusion , biology , veterinary medicine , mycobacterium , medicine , virology , immunology , pathology , tuberculosis , mycobacterium tuberculosis , antiserum
Summary The diagnosis of subclinical paratuberculosis is still considered a major problem worldwide. As part of investigating diagnostic strategies for the paratuberculosis infection, sequential results of various diagnostic methods in a progressive experimental infection in goats were evaluated. Twenty‐three goat kids were divided into three groups: the infected, contact and control, comprising 10, five and eight goats respectively. Animals of the infected group were orally inoculated on seven occasions with 5 ml of inoculum containing 2 × 10 9 Mycobacterium avium ssp. paratuberculosis per ml. Lymphoycte proliferation test using johnin PPD detected paratuberculosis infection from 60 days post‐infection (DPI) onwards. The johnin PPD was found to be a better antigen for the proliferative assays as compared with the sonicated antigen. The faecal smear examination with acid‐fast staining detected more goats as positive than bacterial culture and polymerase chain reaction (PCR). Lipoarabinomannan enzyme‐linked immunosorbent assay (ELISA) started detecting infected goats from 150 DPI onwards followed by indirect ELISA and agar gel immunodiffusion from 180 DPI onwards. Histological examination was confirmatory and detected five infected goats as positive.