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Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental mineral trioxide aggregate
Author(s) -
Pereda G Obando,
Fudinaga AC Cornejo,
Beltrán H Salas,
Peroni LA,
StachMachado D
Publication year - 2012
Publication title -
australian dental journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 71
eISSN - 1834-7819
pISSN - 0045-0421
DOI - 10.1111/j.1834-7819.2012.01701.x
Subject(s) - mineral trioxide aggregate , rankl , gene expression , viability assay , dentistry , macrophage , osteoclast , inflammatory response , protein expression , tumor necrosis factor alpha , microbiology and biotechnology , chemistry , cell , inflammation , andrology , medicine , in vitro , gene , immunology , biology , biochemistry , activator (genetics)
Background: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non‐surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF‐α, IL‐1β and IL‐6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Methods: Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental‐ and commercial‐grey MTA co‐cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. Results: The expression of TNF‐α for both commercial and experimental MTA was higher, while the expression of IL‐1β and IL‐6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. Conclusions: This study suggests that commercial and experimental MTA promotes anti‐inflammatory processes, as well as bone healing capacity.