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Cytotoxicity of polymerized commercial cyanoacrylate adhesive on cultured human oral fibroblasts
Author(s) -
Thumwanit V.,
Kedjarune U.
Publication year - 1999
Publication title -
australian dental journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 71
eISSN - 1834-7819
pISSN - 0045-0421
DOI - 10.1111/j.1834-7819.1999.tb00228.x
Subject(s) - cyanoacrylate , crystal violet , adhesive , cytotoxicity , dentures , staining , incubation , toxicity , chemistry , acrylic resin , polymerization , fibroblast , mtt assay , dentistry , medicine , cell , biochemistry , in vitro , pathology , polymer , organic chemistry , layer (electronics) , coating
Cyanoacrylate (CA) has been used as both a commercial and tissue adhesive. Dentists may have had the experience of patients repairing their own acrylic‐based dentures using a cyanoacrylate (CA) adhesive known as ‘super glue’. This study evaluated the cytotoxicity of commercial CA adhesives when fully polymerized, as well as the toxicity of substances released from polymerized commercial CA adhesives after incubation of these materials for various periods of time. Toxicity was tested on cultured oral fibroblasts. Dead cells found around the various CA‐coated filter papers constituted inhibitory zones which varied from 200–1000 μm and which persited for two weeks. Control oral fibroblasts grew to approach the waxcoated filter paper. Cell viability testing using MTT and crystal violet staining methods supported the conculsion that polymerized CA‐coated filter paper released substances that are toxic to cells, while wax‐coated filter paper gave the same result as the control. The crystal violet staining method was also used to investigate the cytotoxicity of various CA materials after incubation for one, three, seven and 14 days and showed that CA continued to release cytotoxic substances at about the same level for at least two weeks. It can be concluded that, if CA adhesive is used for repair of broken dentures, it will release substances which are toxic to human oral fibroblast cells. This release of substances may persist for at least two weeks.