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A preliminary study of the effects of laser radiation on collagen metabolism in cell culture
Author(s) -
Skinner S. M.,
Gage J. P.,
Wilce P. A.,
Shaw R. M.
Publication year - 1996
Publication title -
australian dental journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 71
eISSN - 1834-7819
pISSN - 0045-0421
DOI - 10.1111/j.1834-7819.1996.tb04854.x
Subject(s) - fibroblast , biostimulation , laser , connective tissue , cell culture , cell , chemistry , dna synthesis , andrology , medicine , microbiology and biotechnology , endocrinology , biology , biochemistry , pathology , dna , in vitro , optics , physics , bioremediation , bacteria , genetics
A low power Ga‐As pulse laser was used to stimulate cultured human embryonic fibroblast cells. Energy fluencies varied from 0–1 J/cm 2 over a period of 1–4 days. Fibroblast procollagen production was monitored by the synthesis of [ 3 H] hydroxy‐proline, and DNA replication was assessed by [ 3 H] thymidine incorporation. Following laser treatment, controlled pepsin digestion measured the increase in cell biostimulation. Maximum increase in collagen production and cell biostimulation occurred after 4 episodes of laser treatment at 24‐hour intervals. Laser doses between 0.099 and 0.522 J/cm 2 had the most significant stimulatory effects on fibroblast function. Clinical efficacy of the low power Ga‐As pulse laser may be related to enhanced connective tissue repair.