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The preparation of autologous fibronectin for use in periodontal surgery
Author(s) -
Thompson E. W.,
Seymour G. J.,
Whyte G. J.
Publication year - 1987
Publication title -
australian dental journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 71
eISSN - 1834-7819
pISSN - 0045-0421
DOI - 10.1111/j.1834-7819.1987.tb01270.x
Subject(s) - fibronectin , polyacrylamide gel electrophoresis , chemistry , medicine , affinity chromatography , dentistry , polyacrylamide , chromatography , surgery , biochemistry , polymer chemistry , enzyme , extracellular matrix
A simple method for the preparation of autologous fibronectin and its subsequent use in periodontal surgery is presented. Fibronectin was isolated from autologous plasma using gelatine mediated affinity chromatography which afforded a yield of 76.5 ± 3.3 per cent. The fibronectin was concentrated to 5 mg/mL to produce a viscosity suitable for use in periodontal surgery. The purity and stability of the fibronectin was assessed by SDS polyacrylamide gel electrophoresis. It was found to contain no contaminants and to remain relatively stable at 4°C over a four to five‐week period.