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Changes in elongation factor‐1α transcripts are uncoupled to changes in EF‐1δ during sea urchin development
Author(s) -
Delalande Christelle,
Monnier Annabelle,
Cormier Patrick,
MulnerLorillon Odile,
Bellé Robert
Publication year - 1998
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1998.tb01074.x
Subject(s) - biology , blastula , sea urchin , human fertilization , elongation , elongation factor , embryogenesis , paracentrotus lividus , messenger rna , microbiology and biotechnology , gene expression , gene , embryo , genetics , rna , gastrulation , ribosome , materials science , ultimate tensile strength , metallurgy
A probe for Sphaerechinus granularis EF‐1α mRNA was generated and sequenced, and further used for comparative developmental analysis of gene expressions of two components of elongation factor‐1, EF‐1α and EF‐1δ. EF‐1α mRNA, detected as a single 2.7 kb transcript decreased 6 h after fertilization and dramatically increased 20 h post‐fertilization at the late blastula stage. The relative ratio of EF‐1α versus EF‐1δ transcripts varied from 0.2 up to 10 (× 50) during development, thus demonstrating uncoupling in the genetic expressions of the two EF‐1 components during the rapid cell divisions following fertilization and in the slow cell cycle period of later development.