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The complete sequence and expression patterns of the atrial myosin heavy chain in the developing chick *
Author(s) -
Oana Shinji,
Machida Shuichi,
Hiratsuka Eriko,
Furutani Yoshiyuki,
Momma Kazuo,
Takao Atsuyoshi,
Matsuoka Rumiko
Publication year - 1998
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1998.tb01069.x
Subject(s) - biology , complementary dna , myosin , open reading frame , peptide sequence , nucleic acid sequence , microbiology and biotechnology , gene , clone (java method) , major histocompatibility complex , cloning (programming) , myh7 , somite , molecular cloning , genetics , embryogenesis , myosin light chain kinase , computer science , programming language
We have earlier reported partial cloning of a cDNA of a chick atrial myosin heavy chain (MHC) gene, CCSV2 and its expression pattern in embryonic chick hearts (Oana et al (1995) Eur J Cell Biol 67, 42–49). In this study, five overlapping cDNA clones (including CCSV2) which together encode the entire open reading frame of the chick atrial MHC gene were characterized, and both the entire nucleotide sequence consisting of 5825 bases and the deduced amino acid sequence consisting of 1931 amino acids determined. Reinvestigation of the nucleotide sequence of the previously reported and presumably different chick atrial specific MHC cDNA clone, AMHC1 (Yutzey et al (1994) Development 120, 871–883), revealed that our clone and AMHC1 encoded the same MHC. The chick atrial MHC gene was strongly expressed in developing chick atria from a very early stage (Hamburger and Hamilton stage 9, 29–33 h) to the adult stage. This gene was also expressed, although weakly, in the ventricle, somite (the precursor to skeletal muscle) and skeletal muscle during embryonic stages but not in adults.