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Behavior of cyclin B and cyclin B‐dependent kinase during starfish oocyte meiosis reinitiation: Evidence for non‐identity with MPF
Author(s) -
Picard André,
Peaucellier Gérard
Publication year - 1998
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1998.tb01058.x
Subject(s) - starfish , biology , maturation promoting factor , cyclin dependent kinase 1 , microbiology and biotechnology , oocyte , cyclin b , cyclin , kinase , meiosis , genetics , cell cycle , ecology , embryo , gene
We reconsider the notion of MPF initiation, which is generally viewed as the prerequisite production of a small cdc2‐cyclin B kinase activity sufficient to trigger its own self‐amplification. Using starfish oocytes as a tool, we show that H1 kinase activation is not explosive, but rather is a gradual process in both time and space. We show further that the production of even a large H1 kinase activity, either in MPF‐microinjected or in hormone‐stimulated oocytes, is not sufficient to trigger MPF amplification.