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Influence of genistein (4′,5,7‐trihydroxyisoflavone) on the growth and proliferation of testicular cell lines
Author(s) -
KumiDiaka James,
Rodriguez Rosanna,
Goudaze Gould
Publication year - 1998
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1998.tb01044.x
Subject(s) - genistein , apoptosis , biology , cell growth , in vitro , cell culture , growth inhibition , microbiology and biotechnology , cancer research , andrology , endocrinology , biochemistry , genetics , medicine
The effects of genistein on testicular cells, TM3, TM4, and GC‐1 spg, were studied in vitro . First, each cell line was cultured with pre‐determined concentrations of genistein for a maximum of 72 h to assess the effects of genistein on in vitro growth of the test cells. A second series of experiments were performed to determine the degree of genistein‐induced apoptosis in these cells, using Apop‐Tag R kit reagents, to detect apoptotic cells in situ by specific end labeling, and detection of DNA fragments produced by the apoptotic process. The results obtained indicate that: i) genistein inhibits the growth and proliferation of testicular cells; ii) growth inhibition and proliferation is dose‐ and exposure‐time dependent; iii) there is significant difference in sensitivity of the different testicular cells to genistein; iv) genistein induces apoptosis in testicular cells in a concentration‐dependent manner. Genistein‐induced apoptosis identifies genistein as a potential diagnostic and therapeutic tool in testicular pathophysiological research.