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Membrane stress causes inhibition of water channels in brush border membrane vesicles from kidney proximal tubule
Author(s) -
Soveral Graca,
Macey Robert I,
Moura Teresa F
Publication year - 1997
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1997.tb01023.x
Subject(s) - brush border , vesicle , biophysics , tonicity , membrane , osmotic pressure , biology , proximal tubule , apical membrane , osmole , osmotic shock , tubule , osmotic concentration , hydrostatic pressure , kidney , biochemistry , endocrinology , physics , gene , thermodynamics
Brush border membrane vesicles (BBMV) from rabbit kidney proximal tubule cells, prepared with different internal solute concentrations (cellobiose buffer 13, 18 or 85 mosM) developed an hydrostatic pressure difference across the membrane of 18.7 mosM, that causes a membrane tension close to 5 × 10 −5 N cm −1 . When subjected to several hypertonic osmotic shocks an initial delay of osmotic shrinkage (a lag time), corresponding to a very small change in initial volume was apparent. This initial osmotic response, which is significantly retarded, was correlated with the initial period of elevated membrane tension, suggesting that the water permeability coefficient is inhibited by membrane stress. We speculate that this inhibition may serve to regulate cell volume in the proximal tubule.