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Lectin binding pattern and band 3 localization in toad skin epithelium and the effect of salt acclimation
Author(s) -
Katz Uri,
Zaccone Gino,
Fasulo Salvatore,
Mauceri Angela,
Gabbay Shoshanah
Publication year - 1997
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1997.tb01002.x
Subject(s) - toad , biology , lectin , xenopus , microbiology and biotechnology , polyclonal antibodies , biophysics , biochemistry , antibody , immunology , endocrinology , gene
Seven lectins were employed to localize glycoconjugates in the skin of a toad (Bufo viridis). Each of the lectins exhibited a particular, specific and selective binding pattern. Peanut lectin (PNA) and WGA bound to mitochondria‐rich (MR) cells, but WGA bound also abundantly, in the dermis. Band 3‐like protein, as indicated by the reaction with polyclonal anti band 3 antibody, was localized exclusively in MR cells. Ionic acclimation (200 mmol/L NaCl, or 50 mmol/L KCl) affected profoundly the binding pattern of the lectins. High NaCl acclimation resulted also in diminishing anti band 3 antibody binding, whereas in skins of KCl‐acclimated toads the staining remained similar to the control. The binding of WGA but not PNA, corresponded with the same cells that stained with anti band 3 antibody. PNA in concentration of < 10 μg/mL reduced reversibly, both the resting and activated Cl − conductance by 25–30%. Based on differential binding of band 3, WGA and PNA, these observations provide conclusive verification of the presence of at least two populations of MR cells in the toad skin epithelium. It is suggested that the PNA positive MR cells may correspond to a β‐type MR cell. The information can be used to study molecular mechanisms that are involved in ionic acclimation.