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Osmium ammine‐B and electron spectroscopic imaging of ribonucleoproteins: Correlation of stain and phosphorus
Author(s) -
Olins Ada L,
Olins Donald E,
BazettJones David P
Publication year - 1996
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1996.tb00976.x
Subject(s) - osmium tetroxide , osmium , nucleic acid , feulgen stain , staining , biology , stain , endoplasmic reticulum , ribonucleoprotein , phosphorus , biophysics , biochemistry , dna , electron microscope , chemistry , rna , optics , organic chemistry , physics , genetics , ruthenium , gene , catalysis
Summary— Ultra‐thin sections of Chironomus salivary glands were stained in a non‐Feulgen procedure with osmium ammine‐B and imaged at several electron energy‐loss windows. For two types of RNP‐containing structures ( ie Balbiani ring granules and endoplasmic reticulum), a significant spatial correlation was observed between stain distribution and net phosphorus distribution. Non‐Feulgen osmium ammine‐B staining does not require the use of ultra‐thin sections and can approximate the distribution of nucleic acid phosphorus.