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A mutation ( dosach ) in Drosophila which affects aster formation and nuclear migration during cleavage
Author(s) -
Craig Sarah S,
Brink Neil G
Publication year - 1996
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1996.tb00965.x
Subject(s) - biology , centrosome , microbiology and biotechnology , cleavage (geology) , advanced spaceborne thermal emission and reflection radiometer , mitosis , mutant , microtubule , spindle apparatus , spindle pole body , chromosome segregation , astral microtubules , drosophila melanogaster , multipolar spindles , kinetochore , genetics , cell division , chromosome , cell cycle , cell , satellite , paleontology , fracture (geology) , gene , engineering , aerospace engineering
Summary— Here we describe a new mutant, dosach ( dos ), in Drosophila melanogaster . In the mutant, centrosomes divide and initiate spindle formation similar to that seen in wild‐type embryos. Nevertheless, mutant embryos form cleavage spindles that lack visible asters and display abnormal morphology, including mono‐ and tri‐polar spindles, spindle chains and incorrect alignment. Irregular nuclear migration is also observed in mutant embryos, and this may suggest that astral microtubules are important for spindle spacing during cleavage and also in maintaining the integrity of the mitotic apparatus. Confocal microscopy has been used to correlate organization of microtubules, centrosomal proteins and chromosomes in wild‐type and dosach ( dos ) embryos.