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Cyclin B (p56 cdc13 ) localization in the yeast Schizosaccharomyces pombe : An ultrastructural and immunocytochemical study
Author(s) -
Audit Muriel,
Barbier Michèle,
SoyerGobillard MarieOdile,
Albert Marie,
Géraud MarieLine,
Nicolas Gisèle,
Lenaers Guy
Publication year - 1996
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1996.tb00949.x
Subject(s) - schizosaccharomyces pombe , biology , microbiology and biotechnology , cyclin dependent kinase 1 , cyclin dependent kinase , cytoplasm , cyclin a , cell cycle , cyclin , interphase , schizosaccharomyces , mitosis , yeast , cell , saccharomyces cerevisiae , biochemistry
Summary— The eucaryote cell cycle is driven by a set of cyclin dependent kinases (CDKs) associated to cyclins, which confer not only the activity but also the substrate specificity and the proper localization of the kinase activity. In the fission yeast Schizosaccharomyces pombe , only one cyclin, the product of the cdc13 gene (p56 cdc13 ), is required to be associated with p34 cdc2 , to control the complete cell cycle. Earlier studies have localized this complex mainly in the nucleus and its periphery. Using new improved electron microscopy (EM) technologies, based on high pressure freezing fixation, we refined previous studies, evidencing cytoplasmic localization of p56 cdc13 , in addition to the nuclear localization previously observed. Further immunofluorescence studies, performed on aldehydically fixed cells, confirmed our EM results, emphasizing the major cytoplasmic localization of p56 cdc13 in interphase cells and the relocalization towards the nucleus in mitotic cells, suggesting that the S pombe cyclin B localization is cell cycle‐regulated.