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Serological characterization and partial purification of an Lyt‐1 homolog in tunicate hemocytes
Author(s) -
Negm Hoda I.,
Mansour Mohamed H.,
Cooper Edwin L.
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1991.tb03021.x
Subject(s) - tunicate , biology , monoclonal antibody , glycoprotein , immunofluorescence , population , microbiology and biotechnology , antibody , antigen , immunology , ecology , demography , sociology
Summary— A panel of alloantisera and monoclonal antibodies specific to murine Lyt‐1 allotypic and framework determinants was used in indirect immunofluorescence and FACS analysis to investigate the occurrence of an Lyt‐1 homolog in tunicate (protochordate) hemocytes. Binding assays and quantitative absorption experiments established the expression of Lyt‐1 cross‐reacting determinants on a distinct population of tunicate hemocytes. These determinants were expressed exclusively by cells with the morphological characteristics of hemoblasts and lymphocytes. In a rapid two‐step purification procedure, Lyt‐1 glycoproteins from tunicate hemocytes and C57B1/6 mouse thymocytes were solubilized and partially purified by affinity chromatography using a mAb anti‐Lyt‐1 frame‐work determinant. In both cell types, antigenic activities were associated with a major 67‐kDa component. Our findings suggest an early phylogenetic emergence of an Lyt‐1 homolog at this level of evolution.