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Location of ribosomal genes in CHO cells; in situ hybridization with a non‐isotopic DNA probe on G‐banded chromosomes *
Author(s) -
AzumGelade MarieClaire,
NoaillacDepeyre Jacqueline,
Gas Nicole
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1991.tb03018.x
Subject(s) - biology , microbiology and biotechnology , chromosome , subtelomere , chinese hamster ovary cell , in situ hybridization , genetics , dna , ribosomal rna , chinese hamster , gene , ribosomal dna , karyotype , nucleic acid thermodynamics , hybridization probe , gene mapping , base sequence , cell culture , gene expression , phylogenetics
Summary— A novel in situ hybridization using sulfonated probes is described. This non‐radioactive approach, which employs chemically modified DNA and immunocytochemical procedures, is compatible with pre‐G‐banding and allows a rapid localization of the hybridized sequences on chromosomal spreads with a high spatial resolution. Using this technique was have localised the Chinese hamster ribosomal genes in the telomeric region of ten chromosomes, and among them in the subtelomeric q region of the Z5 chromosome. These results are discussed, the genetic markers confirming and locating the origin of Z group chromosomes by rearrangements of Chinese hamster chromosomes.