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The culture of human skin fibroblasts on microcarriers in the presence of serum substitute: Ultroser G
Author(s) -
Miech Gilles,
Myara Isaac,
Mangeot Monique,
Gautier Marthe,
Lemonnier Alain
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1990.tb00897.x
Subject(s) - microcarrier , biology , cell culture , fibroblast , andrology , human skin , microbiology and biotechnology , immunology , genetics , medicine
Summary— The effects of the substitution of serum by Ultroser G on human skin fibroblasts cultured on microcarriers were analysed. Cultures could not be established on microcarriers in the presence of Ultroser G. However, microcarrier cultures started in the presence of 10% foetal calf serum, and transferred to 2% Ultroser G after 7 days resulted in high cell densities.