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Methionine‐independence, tumorigenicity and oncogene expression of rat hepatocarcinoma cells
Author(s) -
Cassingena Roland,
LafargeFrayssinet Christiane,
Painchault Véronique,
Estrade Simone,
Nardeux Pierre,
Frayssinet Charles
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1990.tb00006.x
Subject(s) - methionine , biology , oncogene , in vitro , transcription (linguistics) , cell culture , in vivo , microbiology and biotechnology , cell , phenotype , reversion , cancer research , biochemistry , amino acid , genetics , cell cycle , gene , linguistics , philosophy
Summary— Methionine‐dependent (Met − Hcy + ) revertant sublines were isolated from a methionine‐independent (Met + Hcy − ) rat hepatocarcinoma cell line (LF). Characterization of these sublines has shown that long‐term culture in methionine‐deprived medium significantly decreases their ability to grow in vitro , their tumorigenicity in new‐born syngeneic animals and their level of transcription of 3 oncogenes (c‐Ki‐ ras , c‐Ha‐ ras , c‐ myc ) involved in hepatic growth. These data strongly support a relationship between methioninedependence and ability to grow in vivo as tumors. A shift in methionine‐supplemented medium for several cell generations of the various sublines grown in methionine deprived medium, has revealed that under these conditions, the cells maintain a diminished in vitro growth and tumorigenic capacity, despite an increased transcription of c‐Ki‐ ras , c‐Ha‐ ras and c‐ myc . This points out the complexity of the relationship between the level of activity of some oncogenes and the expression of the malignant phenotypes.