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Microfilaments and cellular signal transduction: Effect of cytochalasin D on the production of cAMP, inositol phosphates, and on calcium movements in rat parotid glands
Author(s) -
Huleux Claire,
Dreux Catherine,
Imhoff Viviane,
ChambautGuerin AnneMarie,
Rossignol Bernard
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb03010.x
Subject(s) - second messenger system , biology , microfilament , inositol phosphate , inositol , calcium , endocrinology , microbiology and biotechnology , cytochalasin d , cytochalasin b , inositol trisphosphate , calcium in biology , muscarinic acetylcholine receptor , medicine , inositol trisphosphate receptor , stimulation , receptor , signal transduction , intracellular , biochemistry , cytoskeleton , cell
Summary— In rat parotid glands, the involvement of the microfilament system in the cellular signal transmission mechanism was tested by measuring the effect of cytochalasin D (which disturbs the microfilament system) on the production of intracellular second messengers. Cytochalasin D (CD) did not affect unstimulated calcium movements (measured by the 45 Ca efflux technique) or inositol phosphate production or cAMP accumulation. Neither did it modify the generation of intracellular second messengers induced by activation of the cholinergic muscarinic receptor (calcium and inositol phosphates). CD dit not affect the cAMP accumulation induced by the activation of the β‐adrenergic receptor whereas it strongly inhibited the calcium movements induced by activation of the same receptor. These data suggest that, in rat parotid glands, calcium movements, induced by β‐adrenergic receptor stimulation need an intact microfilament system to occur, whereas the muscarinic pathway ( via IP 3 ) does not.