Premium
Transcription of dispersed repeated sequences during Pleurodeles waltl oogenesis
Author(s) -
Randsholt Neel B.,
Muller JeanPierre,
Loones MarieThérèse
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb03005.x
Subject(s) - pleurodeles , biology , transcription (linguistics) , oogenesis , dna , microbiology and biotechnology , genetics , oocyte , rna , in situ hybridization , gene , messenger rna , embryo , amphibian , paleontology , linguistics , philosophy
Summary— Several repeated DNA sequences were isolated from a partial genomic DNA library of the newt Pleurodeles waltl . These repeated DNA elements are dispersed over the 12 P. waltl bivalents, and some of them are transcribed in the oocyte. We describe the localization of label following in situ hybridization to nascent RNA attached to the lateral loops of lampbrush chromosomes. Variations in the number and the location of labelled loops were constantly found for several of the probes. The results are discussed in view of the “cotranscription model” of RNA synthesis on lampbrush chromosomes. We speculate on the possible origins of variation in transcription on lampbrush loops.