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Immunoelectron microscopical identification of the uncoupling protein in brown adipose tissue mitochondria
Author(s) -
Cinti Saverio,
Zancanaro Carlo,
Sbarbati Andrea,
Cicolini Massimo,
Vogel Peter,
Ricquier Daniel,
Fakan Stanislav
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00883.x
Subject(s) - biology , immunocytochemistry , ultrastructure , mitochondrion , uncoupling protein , brown adipose tissue , immunoelectron microscopy , adipose tissue , microbiology and biotechnology , thermogenin , anatomy , biochemistry , immunohistochemistry , endocrinology , immunology
The distribution of the uncoupling protein (UCP) in brown adipocyte mitochondria of the hibernant Muscardinus avellanarius was obtained by ultrastructural immunocytochemistry. In both cryosections and sections of Lowicryl‐embedded material UCP was localized in the mitochondrial cristae of brown adipocytes, but not in liver mitochondria. It should now be possible to easily identify the morphology of cells committed to BAT differentiation in the tissue as well as in cell culture.