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The acentriolar state of the Drosophila cell lines 1182
Author(s) -
Debec Alain,
Abbadie Corinne
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00876.x
Subject(s) - centriole , biology , centrosome , drosophila melanogaster , microbiology and biotechnology , mitosis , drosophila (subgenus) , schneider 2 cells , cell , genetics , cell cycle , gene , rna interference , rna
A Drosophila melanogaster cell line devoid of centrioles has been recently described. In order to achieve an easier characterization of these acentriolar cells, we used the monoclonal antibody Bx 63 of M. Frusch which recognizes the Drosophila centrosome. Although centrosomes are detected at every mitotic pole in Drosophila cells with centrioles, no such structure has been observed in 1182‐4 acentriolar cells. The antigenic material is, however, present in these cells. Moreover, we noticed a certain proportion of acentriolar cells in 4 other 1182 lines. The lack of centrioles previously found only in the 1182‐4 cells seems therefore not accidental and should be linked to their particular origin.