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Are laminin binding sites on tumor cell surface involved in the indomethacin‐induced sensitivity to natural cytotoxic cells?
Author(s) -
Aliño SalvadorFrancisco,
Unda FernandoJosé,
PerezYarza Gorka,
Cañavate MariaLuz
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00842.x
Subject(s) - cytotoxicity , cytotoxic t cell , laminin , biology , effector , cell culture , microbiology and biotechnology , cell , lymphokine activated killer cell , cancer research , immunology , in vitro , biochemistry , interleukin 12 , genetics
Prostaglandins are secreted by a variety of tumor cell lines. The prostaglandin synthesis inhibitor indomethacin (IND) inhibits 3LL tumor growth after both intramuscular or intrasplenic transplantation (45 and 72%, respectively). Moreover, when tumor cells were cultured with IND, the sensitivity of 3LL cells to natural cytotoxic (NC) effector cells was increased (30%) and a higher cytotoxicity was reached when both target and effector cells were treated. This effect was reversed partially or totally when the assay was performed in the presence of laminin or an octapeptide from the laminin B1 chain. In addition, we correlate the increased cytotoxicity mediated by IND with an enhanced ability of 3LL tumor cells to bind labeled laminin (55%). In summary, our results show that the blockage or modulation of cell surface laminin binding components could be directly correlated with the sensitivity of tumor target cells to be eliminated by way of natural cytotoxicity.