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Water permeability in different epithelial barriers
Author(s) -
Capurro C.,
Escobar E.,
Ibarra C.,
Porta M.,
Parisi M.
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00827.x
Subject(s) - permeability (electromagnetism) , toad , chemistry , materials science , medicine , membrane , biochemistry
The water permeability properties of a series of epithelial barriers (the toad urinary bladder [TUB], the rat caecum [RC], the distal human colon [DHC], and the human amnion [HA] were studied in different experimental conditions. Three parameters were simultaneously determined: the water permeability coefficient in the presence of a transepithelial hydrostatic gradient (P hydr ); the water permeability coefficient in the presence of an osmotic gradient (P osm ); and the transepithelial potential difference (dV). All experiments were performed with the same experimental device, allowing comparison of the permeability properties of the barriers tested. The results obtained were: (1) TUB (N=8): P hydr = 0.079±0.008 cm/s; P osm = 0.0004±0.0002 cm/s; dV=31±5 mV; (2) TUB after ADH ( N =8): P hydr = 0.093±0.012 cm/s; P osm = 0.0065±0.0011 cm/s; dV=52±8; (3) RC (N=10): P hydr = 0.18±0.02 cm/s; P osm = 0.0019±0.0004 cm/s; dV=3.9±0.1 mV; (4) RC adapted to a high K diet ( N =10): P hydr = 0.21±0.02 cm/s; P osm = 0.0018±0.0006 cm/s; dV=4.5±0.5 mV; (5) DHC ( N =6): P hydr = 0.22±0.03 cm/s; P osm = 0.002±0.05 cm/s; dV=15±3 mV; (6) HA ( N =10): P hydr = 0.32±0.05 cm/s; P osm = 0.0154±0.0015; dV=0. The results show a good correlation between P hydr and dV, but not between dV and P osm or between P osm and P hydr .