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Nucleologenesis: use of non‐isotopic in situ hybridization and immunocytochemistry to compare the localization of rDNA and nucleolar proteins during mitosis
Author(s) -
JiménezGarcía Luis F.,
Rothblum Lawrence I.,
Busch Harris,
Ochs Robert L.
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00795.x
Subject(s) - nucleolus , fibrillarin , biology , rna polymerase i , microbiology and biotechnology , telophase , in situ hybridization , metaphase , ribosomal dna , nucleolus organizer region , mitosis , ribosomal rna , interphase , immunocytochemistry , anaphase , rna , rna polymerase , messenger rna , genetics , cell cycle , gene , chromosome , phylogenetics , endocrinology , cytoplasm
Using in situ hybridization and immunocytochemistry during interphase and mitosis, we have compared the distribution of ribosomal DNA (rDNA) to that of the nucleolar proteins fibrillarin and RNA polymerase I. During interphase, nucleolar proteins were localized at sites throughout the nucleolus while the bulk of rDNA was localized in a single restricted nucleolar area. During metaphase and anaphase, all six NORs were detected by in situ hybridization, Ag‐staining, or by the immunolocalization of RNA polymerase I. During telophase, rDNA and RNA polymerase I were found in a distinct subset of the prenucleolar bodies (PNBs) which obviously must contain the nucleolar organizers. Other numerous PNBs are smaller in size and do not contain detectable amounts of rDNA or RNA polymerase I. Therefore, reconstruction of the nucleolus originates in telophase‐specific domains which contain both rDNA and RNA polymerase I.