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In vitro growth properties and PCR ras gene analysis of a murine embryonal carcinoma cell line harboring an amplified C‐Ki‐ ras gene
Author(s) -
Vilette Didier,
d'Auriol Luc,
Galibert Francis,
Peries Jorge,
EmanoilRavier Rodica
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00792.x
Subject(s) - biology , microbiology and biotechnology , gene , embryonal carcinoma , plating efficiency , cell culture , doubling time , in vitro , point mutation , cell growth , mutation , cancer research , genetics , cellular differentiation
Ras proto‐oncogenes are thought to be involved in both proliferation and malignant processes. We examined some growth properties of a murine embryonal carcinoma cell line (ECC), PCC4, that have been shown previously to be amplified for the c‐KI‐ ras gene. Our results show that doubling time and plating efficiency are not significantly affected by such amplification. To examine the possible link between malignant behavior and c‐Ki‐ ras alteration, we investigated activating mutations in this PCC4 cell line as well as in other ECC. Analysis of the in vitro amplified Ki‐ras gene by PCR technology has not revealed point mutations in any of the ECC examined.