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Synthesis of frog virus 3 proteins occurs on intermediate filament‐bound polyribosomes
Author(s) -
Murti Kuruganti,
Goorha Rakesh
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00791.x
Subject(s) - biology , polysome , protein filament , virus , microbiology and biotechnology , biophysics , ribosome , virology , biochemistry , rna , gene
Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)‐infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton‐extracted (cytoskeletons), FV3‐infected BHK cells with antivimentin antibodies showed that the major components of the detergent‐resistant residue are the intermediate filaments (IF) and polyribosomes. Double immunogold labeling studies with anti‐FV3 and antivimentin antibodies revealed that FV3 proteins are associated with polyribosomes that are bound to the If network. Biochemical studies of the cytoskeletons prepared from FV3‐infected cells showed that a substantial fraction of all newly synthesized FV3 proteins associate with the cytoskeleton and that the association is not disrupted by colchicine (microtubule‐disrupting drug) or cytochalasin B (microfilament‐disrupting drug). Together the above studies suggest that IF may provide the scaffold for the attachment of polyribosomes that are active in viral protein synthesis.