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Internalization of atrial natriuretic peptide by adrenal glomerulosa cells
Author(s) -
Morel Gérard,
Mesguich Patrick,
Chabot JeanGuy,
BellesIsles Marthe,
Jeandel Lydie,
Heisler Seymour
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00787.x
Subject(s) - internalization , atrial natriuretic peptide , medicine , endocrinology , biology , aldosterone , membrane , golgi apparatus , zona glomerulosa , peptide hormone , angiotensin ii , microbiology and biotechnology , biochemistry , cell , endoplasmic reticulum , hormone , blood pressure
Internalization of 125 I‐labelled atrial natriuretic peptide ([ 125 I]ANP) by rat adrenal glomerulosa cells in vivo was investigated by means of an ultrastructural autoradiographic approach. One to 30 min after IV injection of [ 125 I]ANP, silver grains were found, at the light microscope level, over all glomerulosa cells; coinjection of 20 μg of unlabelled ANP inhibited this binding by 64%. At the electron microscope level, the time‐course study indicated maximal silver grain densities in plasma membranes 1 min after IV injection; grains were detected in mitochondria (external membranes and matrix) 2 min after injection, with maximal labelling at 15 min. The cytoplasmic matrix was labelled only 30 min after injection. During the time‐course, labelling of nuclei, Golgi apparatus, and lysosomes was minimal. The data suggest that after binding to plasma membranes ANP is rapidly internalized and distributed within glomerulosa cells. The association of radioactivity with mitochondria suggests that ANP may have intracellular sites of action complementary to those on plasma membranes.