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Does the synthesis of ribosomal RNA take place‐within nucleolar fibrillar centers or dense fibrillar components?
Author(s) -
Rasˇka Ivan,
Reimer Georg,
Jarni´k Michal,
Kostrouch Zdeneˇk,
Rasˇka Karel
Publication year - 1989
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1989.tb00774.x
Subject(s) - biology , immunocytochemistry , rna , ribosomal rna , microbiology and biotechnology , small nuclear rna , rna polymerase i , dna , polymerase , biophysics , nucleolus , rna polymerase , biochemistry , rna dependent rna polymerase , nucleus , gene , endocrinology
By means of immunocytochemistry performed on cryosections of cultured cells, RNA polymerase I was localized mainly to nucleolae fibrillar centers. The labelling of nucleolar dense fibrillar components was low and depended on the cell type. In contrast, DNA topoisomerase I and RNP complexes containing U3 snRNA were enriched in dense fibrillar components, their occurrence in fibrillar centers being usually much less.