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Comparative determination of the asialoglycoprotein receptor by ligand and antibody binding in hepatocytes from normal and diabetic rats
Author(s) -
Slama Abdelhamid,
Zinbi Hassan,
Feger Jeanne,
Dodeur Michèle
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00761.x
Subject(s) - asialoglycoprotein receptor , receptor , biology , polyclonal antibodies , cell , ligand (biochemistry) , cell surface receptor , antibody , scatchard plot , microbiology and biotechnology , biochemistry , endocrinology , medicine , hepatocyte , immunology , in vitro
The number of cell surface and total asialoglycoprotein receptors was investigated in normal and diabetic rat hepatocytes using 2 methods: ligand and polyclonal antibody binding. An identical number of immunoreactive receptors was found in both types of cells, while the ligand binding activity of cell surface receptors was reduced by 58% in diabetic rats compared with normal ones, or by 33% for total cell receptors.