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Differential response of primary cultures of human and rat hepatocytes to aflatoxin B 1 ‐induced cytotoxicity and protection by the hepatoprotective agent (+)‐cyanidanol‐3
Author(s) -
Bégué JeanMarc,
Baffet Georges,
Campion JeanPierre,
Guillouzo André
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1111/j.1768-322x.1988.tb00756.x
Subject(s) - aflatoxin , biology , cytotoxicity , primary (astronomy) , differential (mechanical device) , differential effects , toxicology , pharmacology , microbiology and biotechnology , biochemistry , in vitro , engineering , endocrinology , physics , astronomy , aerospace engineering
The acute hepatotoxicity induced by aflatoxin B 1 (AFB 1 ) and the potential protective effect of (+)‐cyanidanol‐3 (Catergen®) were evaluated in both human and rat hepatocyted in primary culture. AFB 1 ‐induced acute toxicity was visualized by light microscope observation and quantified by measurement oflactic dehydrogenase activity in the medium. Human hepatocytes were susceptible to AFB 1 ‐induced cytotoxicity but no evident relationship between the concentration of mycotoxin and the extent of cellular damage was established. (+)‐Cyanidanol‐3 was not toxic at concentrations up to 2×10 −3 M, but no obvious protective effect from AFB 1 ‐induced injury was evidenced in human cells. By contrast, rat hepatocytes responded in a dose‐related manner toAFB 1 . (+)‐Cyanidanol was toxic at 10 −3 M, but even at this concentration exerted a strong protective effect against AFB 1 ‐induced cytotoxicity. Such species differences suggest the existence of metabolic differences in both AFB 1 and (+)‐cyanidanol‐3 activating and deactivating mechanisms.